Skip to content

Metagenomic functional annotation of all reads

SOP: Metagenome 3 - Functional annotation with MMSeqs 2


Introduction

Requirements

1. Build or acquire MMSeqs 2 database

2. Generate jobfiles (executables) containing parameters to run MMseqs2

3. Execute jobfiles to generate the functional predictions

4. Pick the top functional hit for each read

Authors

Authors: Robyn Wright, Dhwani Desai, Gina Filloramo & André Comeau
First created: 13 July 2021
Last updated: 16 October 2026
Based on initial versions by: André Comeau (Metagenomics Standard Operating Procedure v3) and Robyn Wright (Microbiome Helper 2 Annotation of reads with MMSeqs2)

Microbiome Helper logo