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Toy/tutorial data

SOP: Toy/tutorial data


This page contains links to the studies that we have taken the tutorial data from, links to download the data, and details on how we have made these files.

Marker gene

We are using samples from this study:

Pascoal, F., Duarte, P., Assmy, P. & Costa, R. (2024) Full-length 16S rRNA gene sequencing combined with adequate database selection improves the description of Arctic marine prokaryotic communities. Annals of Microbiology.

This study contains both full-length (PacBio) and V4-V5 region (Illumina) 16S rRNA gene sequencing data for samples collected from the Arctic Ocean.

If you just want to run through the tutorials, then you can download the data to your laptop/server like so.

Illumina:

mkdir arctic_ocean_illumina/
cd arctic_ocean_illumina/

wget http://kronos.pharmacology.dal.ca/public_files/MH2/marker_gene/illumina/raw_data.tar.gz
wget http://kronos.pharmacology.dal.ca/public_files/MH2/marker_gene/illumina/arctic_study_metadata_illumina.txt

tar -xvf raw_data.tar.gz
rm raw_data.tar.gz

PacBio:

mkdir arctic_ocean_pacbio/
cd arctic_ocean_pacbio/

wget http://kronos.pharmacology.dal.ca/public_files/MH2/marker_gene/pacbio/raw_data.tar.gz
wget http://kronos.pharmacology.dal.ca/public_files/MH2/marker_gene/pacbio/arctic_study_metadata_pacbio.txt

tar -xvf raw_data.tar.gz
rm raw_data.tar.gz

Authors

Authors: Robyn Wright
First created: 19 October 2026
Last updated: 19 October 2026
Based on initial versions by: NA

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